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KMID : 0381920110410010061
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Korean Journal of Microscopy
2011 Volume.41 No. 1 p.61 ~ p.67
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Morphological Study of Storage Granules of Cotyledon Cells in Cannabis sativa cv. Chungsam
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Lee Na-Young
Kim Dong-Min
Kim Eun-Soo
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Abstract
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The purpose of this study is to investigate the hemp (Cannabis sativa cv. Chungsam) seed structure and ultrastructure of food reserves by scanning and transmission electron microscopy. We examined the seed coat and embryo consisting of a hypocotyl-radicle axis and two cotyledons. The seed coat consisted of exotesta and endotesta. The exotesta was a mechanical layer with lignified and elongated cells, while endotesta of the underlying layers of the exotesta was consisted of two separated cell layers. The collapsed outer layer of endotesta showed the unique reticulate structures. In cotyledon cells, protein and lipid bodies occupied most of cytoplasm. Protein bodies varied in diameter from 1.8 to 5.0 μm and possessed a protein matrix containing electron-dense globoid crystals. Numerous lipid bodies ranged from 0.8 to 3.0 μm in diameter were distributed around the protein bodies. During the early stages of breakdown, protein bodies rapidly changed their shape into the granular feature, however, lipid bodies were gradually degradated and fused each other. The degeneration process of protein bodies and lipid bodies of cotyledon cells might be correlated with the reports which hemp seeds rapidly lose their ability to germinate.
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KEYWORD
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Cannabis sativa, Hemp, Cotyledon, Lipid body, Protein Body
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